多囊泡型二氢杨梅素脂质体的制备、表征及其抑菌性能

The emergence of drug-resistant Staphylococcus aureus (S. aureus) and the lack of high-efficiencyantibiotics have threatened food quality and public safety. Therefore, it is urgent to find new treatment strategies toresist the increasingly severe bacterial challenge. In this work, dihydromyricetin (DMY) was obtained by using ahydrothermal extraction technology from Ampelopsis grossedentata. Then, the multivesicular dihydromyricetin-coated liposomes (DMY-lips) were prepared by using liposomes as drug carriers and polyethylene glycol 4000 as modifiers. The successful formation of DMY-lips was determined by UV-vis spectrophotometer, Fourier infraredspectrometer, X-ray powder diffractometer and simultaneous thermal analyzer. Moreover, the multivesicularstructure of DMY-lips was confirmed by transmission electron microscope and dynamic light scattering. Theobtained DMY-lips exhibited a uniform particle size with an average particle size of 155 nm and a drug loading rateof 42.93%. In addition, antibacterial experiments found that DMY coated liposomes will improve drug antibacterialactivity and antibacterial time, which due to liposomes increase the degree of hydrolysis and membrane permeabilityof DMY. The minimum inhibitory concentration of DMY-lips against S. aureus is 0.05 mg/ml. Noteworthy, theresults of biological scanning electron microscopy and electrical conductivity test displayed that DMY-lips candestroy the cell barrier of S. aureus, causing intracellular materials leaked, resulting in bacterial cell death.Therefore, the multivesicular dihydromyricetin-coated liposomes have great potential in the pharmaceuticalindustry and are expected to reduce the medical systemƳs dependence on chemical antibiotics.