剧毒卡尔藻与东海原甲藻间的化感作用研究

Potential allelopathic interaction between Karlodinium veneficum and Prorocentrum donghaiense was investigated in bi-algal culture and culture filtrate in nutrient-replete medium. The results show that the cell-free filtrate of the K. veneficum culture in later exponential growth phase inhibited the growth of P. donghaiense apparently. In contrast, the filtrate of P. donghaiense exerted no allelopathic activity to K. veneficum, demonstrating that the release of allelopathic compounds inhibited the growth. Furthermore, the cultures of K. veneficum in later exponential growth phase were harvested and loaded onto a RP column using C18, and eluted with a step gradient consisting of different percentage of MeOH. The results show that 80% MeOH fraction depressed P. donghaiense growth more obviously. Further studies are needed to explore allelochemicals released by K. veneficum.