Abstract
Immobilizing proteins on gold surface is very important for the immunological detection with Surface Plasmon Resonance Sensors. It is practical to investigate how to validate that a method can effectively immobilize proteins on the gold surface. A method using enzyme (IgG-HRP) as a flag is presented. If a method can immobilize proteins, it can also immobilize enzyme. The IgG-HRP linked gold surface is treated with the solution of TMB and H2O 2. The spectrum of the solution can tell whether the enzyme is successfully immobilized and whether that method can effectively immobilize proteins. Terminate the reaction with HCl, there will be a peak at 450nm if the IgG-HRP is indeed immobilized. One immobilizing method was tested in this way. Anti-IgG was immobilized with this tested method, and the result was satisfied after the IgG was combined.
| Translated title of the contribution | Simple way to validate the efficiency of immobilization of proteins on gold surface in SPR sensor |
|---|---|
| Original language | Chinese (Simplified) |
| Pages (from-to) | 1477-1480 |
| Journal | Chinese Journal of Sensors and Actuators |
| Volume | 21 |
| Issue number | 9 |
| Publication status | Published - Sept 2008 |
| Externally published | Yes |
Research Keywords
- 表面等离子共振传感器
- 验证蛋白质固定方法
- 酶反应
- Surface plasmon resonance sensor
- validation for immobilizing proteins
- enzyme reaction
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