Plasmonic Photothermal Multiplex Nucleic Acid Amplification
DescriptionNucleic acid amplifications based on polymerase chain reaction (PCR) have shown significant potential as a sensitive and reliable tool for most fields of biological and medical researches. However, there is still a critical need for a new technology and system to address the limitations of the current methodologies of PCR such as long procedural time, heavy and expensive instrumentation and high energy consumption. Here, we propose a novel light- and nanomaterials-based nucleic acid amplifications for fast, quantitative and multiplex semi-real-time PCR, termed plasmonic photothermal multiplex PCR (PPT-mPCR). PPT-mPCR uses self-assembly behaviors of DNA that is tagged on two nanoparticles: one particle with plasmonic and magnetic property for photothermal effect and collecting, the other particle with Raman active molecule for signaling. Primer-modified two nanoparticles can form the assembled structures under the amplification of target DNA by photothermal thermocycling.
|Effective start/end date||1/09/19 → …|