High-resolution Biosensor Assays for Interaction Analysis of Small Molecules with Biomolecular Complexes

Project: Research

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Biosensor technology is in a unique position as a flexible quantitative assay system to systematically evaluate the function of molecular activity. The objective of the project is to develop a series of high-resolution quartz crystal resonator (QCR)-based assays for analysis of the interactions between small molecules with large biomolecular complexes. Initially, these high resolution assays can be used to identify binders from synthetic chemical libraries or natural product libraries and enable ranking of binding stability for lead optimization. Specifically, the focus will be on the development of QCR assays for screening drugs targeting at:polymerase-DNA intertactions related to anti-cancer drug screening;insulin receptor-ligand interactions related to anti-diabetes drug screening; andpyridoxal kinase-substrate interactions related to anti-central nervous system (CNS) disease drug screening.Ultimately, these assays may be used in several different phases of the drug discovery process: from basic research, concept validation and primary screening through to secondary screening and characterization.


Project number9041110
Grant typeGRF
Effective start/end date1/01/0723/09/09