Exploring a Phosphate Signal Transduction Pathway in Pseudomonas Syringae Virulence
DescriptionPseudomonas syringae pathovars are widespread pathogens that infect various staple crops, thus causing huge economic losses and presenting a threat to food security worldwide. Like many other phytopathogenic bacteria, P. syringae deploys its type III secretion system (T3SS) to invade host plants and cause lethal diseases. Although a number of components regulating the T3SS gene expression have been identified, how the bacterium senses the environmental conditions and further transduce the signal(s) to the known regulatory components remain largely unknown. We have recently found a novel two-component system PSPPH5115/5114 that positively regulates T3SS in P. syringae. In our previous studies, we found that the histidine kinase PSPPH5115 sensed phosphate, and its cognate response regulator PSPPH5114 directly bound to promoters of hrpL and hopR1. Interestingly, PSPPH5114 can phosphorylate GltR that is involved in glucose metabolism. We propose a novel signaling pathway of phosphate-PSPPH5115- T3SS/biofilm, whose verification is the focus of the proposal. Genetic and biochemical characterizations of this novel signaling pathway is the first step toward identifying signals to which the T3SS responds, and toward the development of new strategies for disease control based on T3SS manipulation. Our long-term goal is to leverage the outcomes of the herein proposed studies to develop better therapies against P. syringae infection.
|Effective start/end date||1/01/23 → …|