Dual-modality Photoacoustic and Fluorescence Imaging with Local-thermal-enhanced Upconversion Nanoparticle
DescriptionDual-modality photoacoustic and fluorescence imaging can provide accurately co-registered complementary optical absorption and fluorescence contrasts across multiple biological scales from organelles to organs. However, conventional dual-modality imaging systems require complex optical path designs, which not only increase the cost, but also weaken the stability of the systems. Herein, we propose a new dual-modality photoacoustic and fluorescence imaging technique that greatly simplifies the imaging system and enhances the imaging contrast via using a novel dual-contrast nanoparticle probe. The new imaging probe is a hybrid nanoparticle consisting of a silica-coated metal nanosphere attached by rare earth doped upconversion nanocrystals (UCNCs), which can offer both photoacoustic and fluorescence contrasts. In particular, the upconversion fluorescence efficiency of the UCNCs can be significantly enhanced by photoacoustic excitation through plasmon-induced local heating. Based on this novel imaging agent, we will develop a dual-modality photoacoustic and fluorescence imaging system that has a simplified structure, high signal to noise ratio, and rich endogenous and exogenous contrasts. The designed hybrid nanoparticle can offer not only frequency-upconverted photoluminescence and photoacoustic signal for dual-modality imaging, but also afford photodynamic therapy and photothermal therapy abilities for efficient treatment. We believe this proposal will pave a new road for building high-efficiency photoacoustic/fluorescence imaging systems.
|Effective start/end date||30/06/21 → …|