Design, Synthesis and Biological Application of Two-photon Fluorescent Probes for Detecting β-secretase

Project: ResearchARG

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β-secretase is an aspartic protease that has been identified as a therapeutic target of Alzheimer’s disease (AD). Literature has reported that increased level of β-secretase can lead to increased formation of β-amyloid and cause early onset of AD. We envision that developing chemical tools for selective detection of β-secretase activity will be helpful to further our understanding of the roles played by β-secretase in the pathogenesis of AD. It can also help in early diagnosis of AD patients. In this study, we aim to develop novel two-photon fluorescent probes for detecting β-secretase. Compared with one-photon imaging, two-photon imaging has a number of prominent properties, such as improved spatial resolution, reduced photobleaching, decreased photodamage and increased imaging depth. We will develop a series of two-photon fluorescent probes with different peptide sequences to detect β-secretase. The sensitivity and the selectivity of the probes toward β-secretase will be thoroughly studied. In addition, the probes will be applied to live cell imaging studies and cell model study of Alzheimer’s disease. Through the proposed study, we aim to develop dynamic and robust chemical tools to further elucidate the biological roles of β-secretase.


Effective start/end date1/06/17 → …