Automated 3D Cell Patterning by Optically-induced Micro Manipulation and Fabrication

Project: Research

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Cell patterning is an essential technique for cell culture at specific environment, which issignificant for the study of cell growth, drug test, tissue regeneration and so on.Comparing to the traditional cell culture in 2D, 3D cell patterning provides a moreaccurate approach for cell biology research, because 3D culture environment is muchmore closely to cells’ living condition in vivo. However, current 3D cell patterningtechniques has the drawbacks of low flexibility or low productivity. Moreover, they aredifficult, if not impossible, to culture the cells selectively, that to select the particularcells and then to culture them in 3D.This project aims to propose a novel 3D cell patterning method to address the aboveissues. This method is based on optically-induced micro manipulation and fabrication,which will enable the selective, flexible and productive 3D cell patterning automatically.First, an optically-induced microchip will be developed and integrated to our currentoptically-induced dielectrophoresis (ODEP) manipulation system. Then, we will transferthe particular cells to the target positions based on ODEP manipulation. After that, wewill generate the hydrogel to embed the selected cells based on electrodeposition bycontrolling the light pattern, DC voltage and the duration time. Finally, We will developa control system with human-machine interface to implement the above 3D cellpatterning process automatically.This project will derivate a novel 3D cell patterning system by integrating themicromanipulation and electro deposition patterning techniques together. In this project,the cell patterning is controlled by the light projection and the applied voltage, throughwhich ensures the high flexibility and high productivity. In addition, this method allowsthe selectively cell patterning based on ODEP manipulation, which will benefit the in-depthunderstanding of the single cell growth and the interaction between differenttypes of cells. Moreover, the visible light, rather than UV light or laser, is used togenerate the hydrogel, which will provide a more safety fabrication process for cellanalysis. Last but not least, the 3D cell patterning process can be performedautomatically, which will not only improve the working efficiency, but will also promotethe wide application of this method potentially. We believe the outcome of this researchwill greatly benefit the basic cell biology, tissue engineering, medical development andother related fields.?


Project number9042218
Grant typeGRF
Effective start/end date1/12/1525/11/19

    Research areas

  • Micro-manipulation,Micro robotic control,Cell manipulation,Micro-fabrication,