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Transcriptomic study reveals molecular mechanism of ciguatoxin production in Gambierdiscus balechii

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Transcriptomic study reveals molecular mechanism of ciguatoxin production in Gambierdiscus balechii
Many benthic dinoflagellates of the genus Gambierdiscus are capable of producing ciguatoxins (CTXs) with polyether structures, which can be possibly bio-accumulated, bio-transformed and bio-magnified in marine food chains. Humans consuming coral reef fishes that have accumulated CTXs from ingested algal food sources might suffer ciguatera fish poisoning. In order to reveal molecular mechanism of CTXs synthesis, we conducted transcriptome sequencing for both CTX-producing and non-CTX-producing Gambierdiscus balechii. Cultures of CTX-producing strain G. balechii (M1M10) and non-CTX-producing G. balechii (T6PRGD03N) were set up in L1 medium. 114,314 unigenes were generated using next generation sequencing via the BGISEQ-500 sequencer. Among them, 84642 (19.61% in total) genes showed differential expression. Polyketides-related sequences were found in the differential expressed gene dataset. The function of these genes includes keto reductase, acyltransferase, dehydratase, enoyl reductase and acyl carrier protein. Differential expression patterns of these transcripts were also analyzed. These data suggested that polyketide synthase might contribute to polyketide synthesis in this dinoflagellates, which also shed light on the study of toxin production mechanism of dinoflagellates.

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TitleTranscriptomic study reveals molecular mechanism of ciguatoxin production in Gambierdiscus balechii
Date21/10/1826/10/18