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Transcriptomic study on the Gambierdiscus balechii reveals the molecular basis of ciguatoxin production

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Transcriptomic study on the Gambierdiscus balechii reveals the molecular basis of ciguatoxin production
Many benthic dinoflagellates of the genus Gambierdiscus are capable of producing ciguatoxins (CTXs) with polyether structures, which can be possibly bio-accumulated, bio-transformed and bio-magnified in marine food chains. Humans consuming coral reef fishes that have accumulated CTXs from ingested algal food sources might suffer ciguatera fish poisoning. Here, we conducted transcriptome sequencing for Gambierdiscus balechii to understand the molecular mechanism of CTX production. Cultures of CTX-producing strain G. balechii (M1M10) and non-CTX-producing strain G. balechii (T6PRGD03N) were set up in L1 medium. Differential gene expression of the two strains was elucidated using the next generation sequencing via the BGISEQ-500 sequencer, which produced 24,140,000 raw reads for each strain. After filtration, these reads were assembled into 114,314 sequences altogether. Sequences were subjected to Basic Local Alignment Search Tool search, annotated with non-redundant (nr) database. Kallsio was applied to calculate read count for each unigene and totally 19.61% of the genes were differentially expressed. Polyketides-related sequences were found, including beta-ketoacyl-CoA synthase, keto reductase, acyltransferase, dehydratase, enoyl reductase and acyl carrier protein sequences. The expression of these genes between M1M10 and T6PRGD03N is being analyzed. These data lend support to the idea that polyketide synthase may contribute to polyketide synthesis in dinoflagellates. This dataset provides important background to future research in order to understand the complex mechanism of toxin production in this dinoflagellate.

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TitleTranscriptomic study on the Gambierdiscus balechii reveals the molecular basis of ciguatoxin production
Date18/05/1820/05/18